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CARBONLETT, vol. 23, no. 1, pp.38-47, 2017

DOI: http://dx.doi.org/ DOI:10.5714/CL.2017.23.038

Electrochemical determination of chloramphenicol using a glassy carbon electrode modified with dendrite-like Fe3O4 nanoparticles

Krishnan Giribabu1,*, Sung-Chan Jang1,2,*, Yuvaraj Haldorai3, Muruganantham Rethinasabapathy1, Seo Yeong Oh1, Arunkumar Rengaraj1, Young-Kyu Han3, Wan-Seob Cho4,♠, Changhyun Roh2,5,♠, and Yun Suk Huh1,♠

Affiliation: 1Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Incheon 22212, Korea
2Biotechnology Research Division, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup-si, Jeonbuk 56212, Korea
3Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Korea
4Laboratory of Toxicology, Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Korea
5Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology (UST), Daejeon 34113, Korea

Abstract: In this study, magnetite (Fe3O4) nanoparticles were electrochemically synthesized in an aqueous electrolyte at a given potential of -1.3 V for 180 s. Scanning electron microscopy revealed that dendrite-like Fe3O4 nanoparticles with a mean size of < 80 nm were electrodeposited on a glassy carbon electrode (GCE). The Fe3O4/GCE was utilized for sensing chloramphenicol (CAP) by cyclic voltammetry and square wave voltammetry. A reduction peak of CAP at the Fe3O4/GCE was observed at 0.62 V, whereas the uncoated GCE exhibited a very small response compared to that of the Fe3O4/GCE. The electrocatalytic ability of Fe3O4 was mainly attributed to the formation of Fe(VI) during the anodic scan, and its reduction to Fe(III) on the cathodic scan facilitated the sensing of CAP. The effects of pH and scan rate were measured to determine the optimum conditions at which the Fe3O4/GCE exhibited the highest sensitivity with a lower detection limit. The reduction current for CAP was proportional to its concentration under optimized conditions in a range of 0.09-47 μM with a correlation coefficient of 0.9919 and a limit of detection of 0.09 μM (S/N=3). Moreover, the fabricated sensor exhibited anti-interference ability towards 4-nitrophenol, thiamphenicol, and 4-nitrobenzamide. The developed electrochemical sensor is a cost effective, reliable, and straightforward approach for the electrochemical determination of CAP in real time applications.

Keyword: magnetite nanoparticles, electrochemical deposition, chloramphenicol, sensor, square-wave voltammetry